Aluminium and cadmium inhibit vitellogenin and its mRNA induction by estradiol-17 beta in the primary culture of hepatocytes in the rainbow trout Oncorhynchus mykiss

Abstract

Effects of Al and Cd on vitellogenin (VTG) and VTG mRNA induction by estradiol-17 beta (E(2)) were examined in primary hepatocyte cultures of rainbow trout. Hepatocytes were precultured for 2 days and then E(2) (2 x 10(-6) M) and Al (10(-6)-10(-4) M) or Cd (10(-9)-10(-6) M) were simultaneously added to the incubation medium. Hepatocytes were cultured for 5 more days. Media and hepatocytes were then analyzed by SDS-PAGE and Northern blotting for VTG and VTG mRNA, respectively. These metals had no appreciable effect on the viability of hepatocytes in culture. However, Al and Cd interfered with VTG production and VTG mRNA expression. Al reduced VTG production in a concentration-dependent way and a significant reduction occurred at Al concentrations greater than 5 x 10(-5) M. VTG mRNA expression also decreased with a negative correlation with Al concentrations (r = -0.98). The inhibition of VTG production by Cd was not concentration-dependent. This metal markedly inhibited VTG production and VTG mRNA expression at 10(-6) M. The Al-induced inhibition of VTG production was restored 7 days after Al removal, but the Cd-induced inhibition was not restored. These results suggest that Al and Cd inhibit VTG production at the transcriptional level to reduce VTG mRNA expression by different mechanisms.