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. 2000 Aug;182(15):4372-4.
doi: 10.1128/JB.182.15.4372-4374.2000.

Identification of the Escherichia coli nicotinic acid mononucleotide adenylyltransferase gene

R A Mehl  1 , C Kinsland, T P Begley
Affiliations

Identification of the Escherichia coli nicotinic acid mononucleotide adenylyltransferase gene

R A Mehl et al. J Bacteriol. 2000 Aug.

Abstract

The gene (ybeN) coding for nicotinate mononucleotide adenylyltransferase, an NAD(P) biosynthetic enzyme, has been identified and overexpressed in Escherichia coli. This enzyme catalyzes the reversible adenylation of nicotinate mononucleotide and shows product inhibition. The rate of adenylation of nicotinate mononucleotide is at least 20 times faster than the rate of adenylation of nicotinamide mononucleotide.

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Figures

FIG. 1
FIG. 1
(A) The YbeN-catalyzed adenylation reaction. (B and C) HPLC analyses of the YbeN-catalyzed adenylation reactions: (B) 1.5 mM NAMN, reaction time is 15 min; (C) 1.5 mM NMN, reaction time is 40 min. (D and E) HPLC analysis of the competitive adenylation of NAMN and NMN: (D) the reaction mixture at t = 5 min; (E) the reaction mixture at t = 0 min. Labeled peaks: 1, NAMN; 2, NMN; 3, ATP; 4, NAAD; 5, NAD.
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