Isozyme characterization of cattle (Bos taurus) and American buffalo (Bison bison) cell cultures
- PMID: 109020
- PMCID: PMC7159466
- DOI: 10.1111/j.1365-2052.1978.tb01431.x
Isozyme characterization of cattle (Bos taurus) and American buffalo (Bison bison) cell cultures
Abstract
Four Bovidae cell lines (BEK-1, MDBK, Bu and EBTr) were characterized by means of enzymatic biochemical markers. Out of 15 enzymatic systems, 3--adenosine deaminase (Ada), phosphoglucomutase (Pgm) and nucleoside phosphorylase (Np)--were found to be polymorphic and quite suitable for biochemical identification of each cell line. The Bu cell line has shown a Np phenotypic pattern which could be distinctive of the Bison bison species.
References
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- Anonymous , 1972. Registry of animal cell lines. American Type Culture Collection.
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- Ansay, M. & Hanset R. 1972a. Soluble glutamic oxaloacetic transaminase (GOT) polymorphism in cattle. Anim. Blood Grps biochem. Genet. 3: 163 – 168.
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- Ansay, M. & Hanset R. 1972c. Purine nucleoside phosphorylase (NP) of bovine erythrocytes: genetic control of electrophoretic variants. Anirn. Blood Grps biochem. Genet. 3: 219 – 227.
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