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. 1975 Jan;77(7):792-4.
doi: 10.1007/BF00799322.

Separation of rosette-forming cells with the aid of an immunosorbent based on sephadex G-25 and G-75

B B Fuks, I V Khazanova, V L Yurin

Separation of rosette-forming cells with the aid of an immunosorbent based on sephadex G-25 and G-75

B B Fuks et al. Bull Exp Biol Med. 1975 Jan.

Abstract

A method of separating rosette-forming cells on columns with an immunosorbent based on sephadex G-75 is described and has been tested. The protein antigen (bovine serum albumin-BSA) is attatched by covalent bonds to the surface of the Sephadex granules oxidized by sodium periodate. Cells with receptors on their surface were tested by the rosette method. Up to 88% of the rosette-forming cells were retained on a column packed with BSA-Sephadex granules. Nonspecific retention of the cells was relatively small, about 4%.

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