Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Jul;29(1):78-81.
doi: 10.2144/00291st01.

Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR green II

C Battaglia  1 , G Salani, C Consolandi, L R Bernardi, G De Bellis
Affiliations
Free article

Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR green II

C Battaglia et al. Biotechniques. 2000 Jul.
Free article

Abstract

A simple, non-destructive procedure is described to determine the quality of DNA arrays before they are used. It consists of a preliminary staining step of the DNA microarray by using SYBR green II, a fluorophore with specific affinity for ssDNA, followed by a laser scan analysis. The surface quality, integrity and homogeneity of each DNA spot of the array can thus be assessed. After this preliminary control, which may avoid further analytical steps that lead to the waste of precious biological samples, a fully reversible staining procedure is performed that produces an array ready for subsequent use.

PubMed Disclaimer

Publication types

LinkOut - more resources