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Review
. 2000 Jul 24;150(2):F45-50.
doi: 10.1083/jcb.150.2.f45.

Genes encoding Drosophila melanogaster RNA polymerase II general transcription factors: diversity in TFIIA and TFIID components contributes to gene-specific transcriptional regulation

N Aoyagi  1 , D A Wassarman
Affiliations
Review

Genes encoding Drosophila melanogaster RNA polymerase II general transcription factors: diversity in TFIIA and TFIID components contributes to gene-specific transcriptional regulation

N Aoyagi et al. J Cell Biol. .
No abstract available

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Figures

Figure 1
Figure 1
RNA pol II and GTF-encoding subunit genes from D. melanogaster, C. elegans, humans, and yeast are grouped by protein complex. Additional information about these genes can be accessed using the indicated gene name or identification number at web sites for the Drosophila (http://www.fruitfly.org/annot/), C. elegans (http://www.wormbase.org/), yeast (http://www.proteome.com/databases/index.html), or human genomes (http://www.ncbi.nlm.nih.gov/). No Gadfly identification has been assigned for Rpb12 because it was not identified by gene prediction programs and no expressed sequence tags have been isolated. However, searches using the human and yeast Rpb12 homologues match translated genomic sequence from EMBL/GenBank/DDBJ accession No. AC017903, and further analysis of sequences surrounding this match reveal additional amino acid similarity that spans 3 exons (our unpublished observation). Each row contains homologous genes from each of the four organisms. An asterisk indicates that the gene is alternatively spliced. ND indicates information that has not been determined. The following identification numbers correlate to predicted mRNAs that in addition to encoding the indicated protein may also encode another protein, presumably due to a gene prediction error: Drosophila CG7150, CG6572, and C. elegans C36B1.3, F43E2.1, Y97E10AR.a, F39H11.2, and Y37E11AL.c. (A) Genes encoding components of RNA pol II, TFIIB, TFIIE, TFIIF, and TFIIH. (B) Genes encoding components of TFIIA and TFIID. Genes in bold typeface have been demonstrated experimentally to be a component of the indicated complex. Genes in normal typeface that are bordered in a black box may be a component of the indicated complex and await experimental evidence. Genes that are not bordered in a black box are components of other complexes. Genes that are expressed in a tissue- or cell type–specific manner are shaded orange, genes that encode components of HAT complexes are shaded pink, and genes that encode sequence similarity to the histone fold motif are shaded blue. A search program that identifies protein motifs (http://www.isrec.isb-sib.ch/software/PFSCAN_form.html) and visual comparison of sequences was used to find the histone fold motifs in TAFIIs and the transcription factors indicated in the text. Yeast BDF1, TAFII48, and TAFII65 are recently described components of TFIID (Sanders and Weil 2000; Matangkasombut et al. 2000; Reese et al. 2000). Yeast TAFII145 and BDF1 display functional and sequence similarity to the NH2 and COOH termini, respectively, of human TAFII250 and therefore are placed in the same box (Matangkasombut et al. 2000). Bdf1 and Bdf2 display sequence similarity but only Bdf1 has been demonstrated to associate with TFIID. TAFII30/ANC1 is also a component of NuA3, TFIIF, and SWI/SNF complexes (John et al. 2000).
Figure 1
Figure 1
RNA pol II and GTF-encoding subunit genes from D. melanogaster, C. elegans, humans, and yeast are grouped by protein complex. Additional information about these genes can be accessed using the indicated gene name or identification number at web sites for the Drosophila (http://www.fruitfly.org/annot/), C. elegans (http://www.wormbase.org/), yeast (http://www.proteome.com/databases/index.html), or human genomes (http://www.ncbi.nlm.nih.gov/). No Gadfly identification has been assigned for Rpb12 because it was not identified by gene prediction programs and no expressed sequence tags have been isolated. However, searches using the human and yeast Rpb12 homologues match translated genomic sequence from EMBL/GenBank/DDBJ accession No. AC017903, and further analysis of sequences surrounding this match reveal additional amino acid similarity that spans 3 exons (our unpublished observation). Each row contains homologous genes from each of the four organisms. An asterisk indicates that the gene is alternatively spliced. ND indicates information that has not been determined. The following identification numbers correlate to predicted mRNAs that in addition to encoding the indicated protein may also encode another protein, presumably due to a gene prediction error: Drosophila CG7150, CG6572, and C. elegans C36B1.3, F43E2.1, Y97E10AR.a, F39H11.2, and Y37E11AL.c. (A) Genes encoding components of RNA pol II, TFIIB, TFIIE, TFIIF, and TFIIH. (B) Genes encoding components of TFIIA and TFIID. Genes in bold typeface have been demonstrated experimentally to be a component of the indicated complex. Genes in normal typeface that are bordered in a black box may be a component of the indicated complex and await experimental evidence. Genes that are not bordered in a black box are components of other complexes. Genes that are expressed in a tissue- or cell type–specific manner are shaded orange, genes that encode components of HAT complexes are shaded pink, and genes that encode sequence similarity to the histone fold motif are shaded blue. A search program that identifies protein motifs (http://www.isrec.isb-sib.ch/software/PFSCAN_form.html) and visual comparison of sequences was used to find the histone fold motifs in TAFIIs and the transcription factors indicated in the text. Yeast BDF1, TAFII48, and TAFII65 are recently described components of TFIID (Sanders and Weil 2000; Matangkasombut et al. 2000; Reese et al. 2000). Yeast TAFII145 and BDF1 display functional and sequence similarity to the NH2 and COOH termini, respectively, of human TAFII250 and therefore are placed in the same box (Matangkasombut et al. 2000). Bdf1 and Bdf2 display sequence similarity but only Bdf1 has been demonstrated to associate with TFIID. TAFII30/ANC1 is also a component of NuA3, TFIIF, and SWI/SNF complexes (John et al. 2000).
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References

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