[5'-methylthioadenosine phosphorylase from the human prostate. 1. Purification and partial characterization]
[Article in
Italian]
- PMID: 109102
Item in Clipboard
[5'-methylthioadenosine phosphorylase from the human prostate. 1. Purification and partial characterization]
[Article in
Italian]
Boll Soc Ital Biol Sper.
.
Abstract
5'-Methylthioadenosine phosphorylase has been purified approximately 340-fold in 20% yield from human prostate: the use of affinity chromatography by Sepharose-Hg has been found particularly advantageous. The enzyme has been partially characterized and an apparent Km of 2.5 x 10(-5) M has been calculated for 5'-methylthioadenosine. The reaction is activated by thiols and shows an absolute requirement for phosphate ions.
Similar articles
-
[5'-methylthioadenosine phosphorylase from Caldariella acidophila. 1. Purification and partial characterization].Boll Soc Ital Biol Sper. 1978 Dec 15;54(23):2355-61. Boll Soc Ital Biol Sper. 1978. PMID: 41533 Italian.
-
Substrate specificity of 5'-methylthioadenosine phosphorylase from human prostate.Biochem J. 1978 Dec 1;175(3):1043-50. doi: 10.1042/bj1751043. Biochem J. 1978. PMID: 105725 Free PMC article.
-
Purification and characterization of 5'-deoxy-5'-methylthioadenosine phosphorylase from human placenta.J Biol Chem. 1986 Sep 15;261(26):12324-9. J Biol Chem. 1986. PMID: 3091600
-
Methylthioadenosine (MeSAdo) phosphorylase deficiency in malignancy.Adv Exp Med Biol. 1988;250:179-85. doi: 10.1007/978-1-4684-5637-0_16. Adv Exp Med Biol. 1988. PMID: 3151225 Review. No abstract available.
-
Physico-chemical and immunological properties of bovine liver 5'-deoxy-5'-methylthioadenosine phosphorylase.Adv Exp Med Biol. 1988;250:187-97. doi: 10.1007/978-1-4684-5637-0_17. Adv Exp Med Biol. 1988. PMID: 3151226 Review. No abstract available.