Human renin mRNA expression and renin activity in transgenic mice

Abstract

The renin-angiotensin system (RAS) plays a very important role in the regulation of blood pressure and electrolyte homeostasis in mammals. It has also been hypothesized to regulate local tissue and organ blood supply in an autocrine/paracrine manner. To further study RAS physiology, transgenic mice carrying the human renin gene were produced by microinjection and were screened by PCR. The human renin transgene was found, by RT-PCR, to be expressed in the heart, kidney, and lung of transgenic mice, but not in the liver or skeletal muscle. The mean levels of renin activity in various tissues were determined by radioimmunoassay. The mean level of renin activity in the plasma and the heart/body weight ratio of the transgenic mice (n=7) were also found to be significantly higher (p< 0.05) than those of the control mice (n= 8). However, no significant differences were seen in the mean levels of renin activities in the kidney and heart between transgenic and control mice. Thus, the transcription of human renin gene in transgenic mice is regulated in a tissue-specific manner. This transgenic model will be useful in clarifying the regulatory mechanisms of the renin gene and the relationship between this gene and diseases.