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. 2000 Aug;66(8):3624-7.
doi: 10.1128/AEM.66.8.3624-3627.2000.

Production of Poly(3-hydroxybutyrate) by fed-batch culture of recombinant Escherichia coli with a highly concentrated whey solution

W S Ahn  1 , S J Park, S Y Lee
Affiliations

Production of Poly(3-hydroxybutyrate) by fed-batch culture of recombinant Escherichia coli with a highly concentrated whey solution

W S Ahn et al. Appl Environ Microbiol. 2000 Aug.

Abstract

Fermentation strategies for the production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes were developed. The pH-stat fed-batch cultures of E. coli CGSC 4401 harboring pJC4, a stable plasmid containing the A. latus PHA biosynthesis genes, were carried out with a concentrated whey solution containing 280 g of lactose equivalent per liter. Final cell and PHB concentrations of 119.5 and 96.2 g/liter, respectively, were obtained in 37.5 h, which resulted in PHB productivity of 2.57 g/liter/h.

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Figures

FIG. 1
FIG. 1
Time profiles of cell concentration (●), PHB concentration (■), residual cell concentration (▴), and PHB content (○) (A) and cell growth rate (●) and PHB synthesis rate (○) (B) during fed-batch culture of E. coli CGSC 4401(pJC4) with feeding solution containing 280 g of lactose equivalent per liter (fermentation C).
See this image and copyright information in PMC

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