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. 1997 May;19(3):170-2.

[Alterations in metastatic properties of hepatocellular carcinoma cell following H-ras oncogene transfection]

[Article in Chinese]
Q Wang  1 , X Feng, Z Lin
Affiliations
  • PMID: 10920888

[Alterations in metastatic properties of hepatocellular carcinoma cell following H-ras oncogene transfection]

[Article in Chinese]
Q Wang et al. Zhonghua Zhong Liu Za Zhi. 1997 May.

Abstract

Objective: To demonstrate the relationship between H-ras oncogene and HCC metastasis.

Methods: Activated H-ras oncogenes were transfected into SMMC7721, a cell line derived from human hepatocellular carcinoma (HCC), by calcium phosphate transfection method. Some metastasis-related parameters were detected in vitro, including adhesion assay, migration assay, expresion of collagenase IV (cIVase) and EGFR.

Results: The abilities of H-ras-transfected cell clones in adhesion to laminin or fibronectin, migration, cIVase secretion increased markedly, and the expression of EGFR elerated moderately. More importantly, these alterations were consistently positive with the expression of p21, the protein product of H-ras oncogene.

Conclusion: H-ras oncogene could induce the metastatic phenotype of HCC cell in vitro, therefore enhancing its metastatic potential.

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