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. 1998 Jan;20(1):25-7.

[Apoptosis of B lymphocytic leukemia induced by anticancer drugs and their cell cycle specificity]

[Article in Chinese]
X Sun  1 , K Saeki, T Masahito
Affiliations
  • PMID: 10921050

[Apoptosis of B lymphocytic leukemia induced by anticancer drugs and their cell cycle specificity]

[Article in Chinese]
X Sun et al. Zhonghua Zhong Liu Za Zhi. 1998 Jan.

Abstract

Objective: Evaluating the effect of apoptosis induced by anticancer drugs on human B lymphocytic leukemia cell lines (Nalm-6) and their cell cycle specificity.

Methods: Nalm-6 cells were treated with various anticancer drugs for 8-24 hours. Apoptotic cells and their cell cycle specificity were measured by using hypodiploid DNA-FCM and TdT assay + DNA staining.

Results: ADR, VP16, CPT, MTX, Ara-C could markedly induce apoptosis of Nalm-6 cells in S phase. CPM, PRD, 6MP were less capable of inducing apoptosis. CPM in high dose resulted in cell necrosis. PRD induced apoptosis in G1 phase, while 6MP induced apoptosis in G1 and S phase. The effect of CPM showed no marked cell cycle specificity.

Conclusion: Hypodiploid DNA-FCM and TdT assay + DNA staining can be used to detect both tumor cell apoptosis and their cell cycle specificity which is helpful to predict prognosis and to design new chemotherapy regimen.

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