Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Aug;38(8):3092-5.
doi: 10.1128/JCM.38.8.3092-3095.2000.

Simple and reliable multiplex PCR assay for surveillance isolates of vancomycin-resistant enterococci

R Kariyama  1 , R Mitsuhata, J W Chow, D B Clewell, H Kumon
Affiliations

Simple and reliable multiplex PCR assay for surveillance isolates of vancomycin-resistant enterococci

R Kariyama et al. J Clin Microbiol. 2000 Aug.

Abstract

Conditions have been optimized for the use of a multiplex PCR for the detection of vancomycin-resistant enterococci in nosocomial surveillance specimens. Seven primer sets targeting the genes vanA, vanB, vanC1, vanC2/C3 Enterococcus faecalis-specific, Enterococcus faecium-specific, and rrs (16S rRNA) were used in one reaction tube. The PCR method developed in the present study is simple and reliable for the rapid characterization of vancomycin-resistant enterococci.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Agarose gel electrophoresis of amplified vanA, vanB, vanC1, vanC2, E. faecalis-specific, E. faecium-specific, and rrs genes by the optimized multiplex PCR assay containing seven primer sets. Lanes: M, 100-bp DNA ladder (New England Biolabs); 1, an E. faecalis vanA isolate; 2, an E. faecalis vanB isolate; 3, an E. faecium vanA isolate; 4, an E. faecium vanB isolate; 5, an E. gallinarum vanC1 isolate; 6, an E. casseliflavus vanC2 isolate; 7, a Pediococcus isolate; 8, a Leuconostoc isolate.
See this image and copyright information in PMC

Comment in

References

    1. Cheng S, McCleskey F K, Gress M J, Petroziello J M, Liu R, Namdari H, Beninga K, Salmen A, DelVecchio V G. A PCR assay for identification of Enterococcus faecium. J Clin Microbiol. 1997;35:1248–1250. - PMC - PubMed
    1. Clark N C, Cooksey R C, Hill B C, Swenson J M, Tenover F C. Characterization of glycopeptide-resistant enterococci from U. S. hospitals. Antimicrob Agents Chemother. 1993;37:2311–2317. - PMC - PubMed
    1. Dutka-Malen S, Evers S, Courvalin P. Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol. 1995;33:24–27. . (Erratum, 33:1434.) - PMC - PubMed
    1. Free L, Sahm D F. Detection of enterococcal vancomycin resistance by multiplex PCR. In: Persing D H, editor. PCR protocols for emerging infectious diseases. Washington, D.C.: ASM Press; 1996. pp. 150–155.
    1. Fujita N, Yoshimura M, Komori T, Tanimoto K, Ike Y. First report of the isolation of high-level vancomycin-resistant Enterococcus faecium from a patient in Japan. Antimicrob Agents Chemother. 1998;42:2150. - PMC - PubMed

MeSH terms

Substances

LinkOut - more resources