Isolation of Legionella oakridgensis from two patients with pleural effusion living in the same geographical area

Abstract

Two cases of Legionnaire's disease caused by Legionella oakridgensis were diagnosed at the university hospital in Nantes, France. The two patients' isolates were identified by means of phenotyping and genotyping methods. Epidemiological investigations concluded that the first case was hospital acquired while the second case was considered community acquired.

FIG. 1

RAPD profiles of L. oakridgensis strains and other Legionella reference strains. Lanes M, molecular weight marker Ready-load 100 bp (Gibco BRL); lane 1, L. oakridgensis ATCC 33761; lane 2, strain 930101868/ATCC 700515 (patient 1, Nantes); lane 3, strain 930101937/ATCC 700516 (patient 2, Nantes); lane 4, strain 88020285; lane 5, strain 88020400; lane 6, strain 88020413; lane 7, strain 88020417 (lanes 4 to 7 are environmental isolates from Nantes hospital); lane 8, L. oakridgensis strain 91020991 (thermal spa Bourbon-Lancy); lane 9, L. oakridgensis strain ATCC 33761; lane 10, L. hackeliae ATCC 35250; lane 11, L. jordanis ATCC 33263; lane 12, L. londiniensis ATCC 49505; lane 13, L. moravica ATCC 43877; lane 14, L. sainthelensi ATCC 35248; lane 15, L. santicrucis ATCC 35301; lane 16, L. spiritensis ATCC 35249.

FIG. 2

IRS-PCR profiles of L. oakridgensis reference strains and isolates. Lanes M, molecular weight marker VI (Boehringer Mannheim); lane 1, strain 89020417; lane 2, strain 88020285; lane 3, 89020400; lane 4, strain 89020413 (lanes 1 to 4 contain isolates from the water distribution system of the Nantes hospital); lane 5, strain 92203624 (from the water distribution system of a Lille hospital); lane 6, strain 930101868/ATCC 700515 (patient 1, Nantes); lane 7, L. oakridgensis strain OR-10/ATCC 33761; lane 8, strain 9020498 (thermal spa, Aix-les Bains); lane 9, strain 930101937/ATCC 700516 (patient 2, Nantes); lane 10, strain 90020551 (from the water distribution system of a Portuguese hospital).