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. 1975 Apr 2;383(4):457-63.
doi: 10.1016/0005-2787(75)90318-4.

A simple method for the preparation of large quantities of pure plasmid DNA

G O Humphreys, G A Willshaw, E S Anderson

A simple method for the preparation of large quantities of pure plasmid DNA

G O Humphreys et al. Biochim Biophys Acta. .

Abstract

Polyethylene glycol quantitatively precipitates plasmid DNA of molecular weight 6-123-10-6, from cleared lysates of plasmid-carrying bacterial strains, After resuspension and density-gradient centrifugation of the precipitated DNA, it is unchanged in length and in transformation efficiency for Escherichia coli K12. Plasmid DNA can be easily prepared in large quantities by including a polyethylene glycol precipitation step in standard plasmid isolation procedures.

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