A Fourier-transform infrared study of the interaction between germ-cell specific sulfogalactosylglycerolipid and dimyristoylglycerophosphocholine

Abstract

In this study, we investigated structural and dynamic changes of sulfogalactosylglycerolipid (SGG) and dimyristoylglycerophosphocholine (DMPC) in a liposomal system (SGG+DMPC, molar ratio 2:3) by Fourier-transform infrared (FTIR) spectroscopy. Cooling of the preheated SGG liposomes (5-65 degrees C) revealed that the liquid crystalline-to-gel phase transition was centered at 45 degrees C. SGG+DMPC liposomes showed a single phase transition at 28 degrees C. Spectral changes of the ester C&z. dbnd6;O groups of SGG and DMPC in the mixed liposomes indicated a decrease in their interfacial hydrogen bonding intermolecularly and with water. Analysis of SGG's symmetric and antisymmetric CH(2) stretching bands revealed that the insertion of DMPC into SGG bilayers increased the number of gauche conformers in SGG's hydrocarbon chains. Overall, the SGG+DMPC liposomes were homogeneous, with reduced interfacial hydrogen bonding and increased orientational and conformational disorder of SGG's hydrocarbon chains.