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. 1975 Mar;72(3):1050-4.
doi: 10.1073/pnas.72.3.1050.

Replication of colicin E1 plasmid DNA added to cell extracts

J I Tomizawa, Y Sakakibara, T Kakefuda

Replication of colicin E1 plasmid DNA added to cell extracts

J I Tomizawa et al. Proc Natl Acad Sci U S A. 1975 Mar.

Abstract

Closed-circular DNA of colicin E1 plasmid can undergo a round of semiconservative replication when added to an extract of Escherichia coli. Extracts of cells that do not carry the plasmid are able to perform complete replication of the plasmid. Replication requires de novo RNA synthesis but not protein synthesis.

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References

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