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. 1975 Mar;72(3):921-5.
doi: 10.1073/pnas.72.3.921.

Interaction of Escherichia coli dnaB and dnaC(D) gene products in vitro

S Wickner, J Hurwitz

Interaction of Escherichia coli dnaB and dnaC(D) gene products in vitro

S Wickner et al. Proc Natl Acad Sci U S A. 1975 Mar.

Abstract

Purified E. coli dnaB and dnaC(D) gene products interact physically and functionally in vitro. This interaction was demonstrated as follows: (a) A complex of dnaB and dnaC(D) gene products was isolated by gel filtration; ATP specifically was required for isolation of the complex. (b) The DNA-independent ribonucleoside triphosphatase activity associated with dnaB gene product was inhibited by dnaC(D) gene product. (c) The dnaC(D) gene product was protected from inactivation by N-ethyl-maleimide by the combination of dnaB gene product and ATP; this protection required ATP specifically.

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