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. 2000 Aug;25(4):375-6.
doi: 10.1038/78040.

Hot-stop PCR: a simple and general assay for linear quantitation of allele ratios

H Uejima  1 , M P Lee, H Cui, A P Feinberg
Affiliations

Hot-stop PCR: a simple and general assay for linear quantitation of allele ratios

H Uejima et al. Nat Genet. 2000 Aug.

Erratum in

  • Nat Genet 2001 May;28(1):97

Abstract

We have developed a simple, quantitative assay for measurement of allele ratios that circumvents the problem of heteroduplex formation skewing the results of restriction endonuclease digestion of PCR products. This assay, 'hot-stop PCR', involves addition of a radiolabelled PCR primer at the final cycle. We applied the assay to analysis of loss of imprinting (LOI) of the insulin-like growth factor II gene (IGF2) in tumours.

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