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. 1999 Dec 15;13(3):215-22.
doi: 10.1385/MB:13:3:215.

Time-resolved fluoroimmunoassay

P Degan  1 , A Podestà, G Montagnoli
Affiliations

Time-resolved fluoroimmunoassay

P Degan et al. Mol Biotechnol. .

Abstract

A method is reported to set up a standard competitive TR-FIA. A simple and inexpensive way to prepare reagents and carry out operations is presented as well, with the aim to make it possible to perform a very sensitive analytical procedure in a personalized way within a nondedicated biochemistry laboratory. This protocol is general and can be easily modified with consideration to the analytical target. Once the antibody is available, both its labeling with diethylenetriaminepentaacetic acid dianhydride and Eu3+, and the setting-up of the assay with measurement of europium ion time-resolved fluorescence in a home-made enhancement solution become feasible.

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References

    1. Anal Biochem. 1984 Mar;137(2):335-43 - PubMed
    1. J Biolumin Chemilumin. 1990 Jul-Sep;5(3):207-12 - PubMed
    1. Anal Chem. 1989 Jan 1;61(1):48-53 - PubMed
    1. Clin Chem. 1987 Dec;33(12):2281-3 - PubMed
    1. J Biol Chem. 1968 Oct 10;243(19):5063-73 - PubMed

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