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. 1975 Jun;28(6):1149-54.

T cell-dependent mediator and B-cell clones

I Lefkovits, J QUintáns, A Munro, H Waldmann

T cell-dependent mediator and B-cell clones

I Lefkovits et al. Immunology. 1975 Jun.

Abstract

Supernatants were collected from keyhole limpet haemocyanin (KLH) primed spleen cells which had been incubated in tissue culture medium with their priming antigen KLH. These non-specific factor (NSF) containing supernatants were then tested in a microculture system for their ability to facilitate an anti-SRBC response of nu/nu or AT times BM spleen cells. It was concluded that:(a) NSF was able to engage a large number of the available pool of sheep erythrocyte (SRBC) specific B cells into an antibody response (b) this response was characterized by the development of clones expressing plaque-forming cells (PFC), the number of PFC produced within a clone being dependent upon the amount of NSF available in that culture; (c) NSF probably acted directly on B cells, and not via other accessory cell types.

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References

    1. J Immunol. 1973 Sep;111(3):900-13 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Feb;71(2):512-6 - PubMed
    1. Immunology. 1975 Jun;28(6):1135-48 - PubMed
    1. Eur J Immunol. 1974 Mar;4(3):164-9 - PubMed
    1. Immunology. 1974 Jul;27(1):53-64 - PubMed

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