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. 1975 Jun;65(3):549-61.
doi: 10.1083/jcb.65.3.549.

Tropomyosin antibody: the specific localization of tropomyosin in nonmuscle cells

E Lazarides

Tropomyosin antibody: the specific localization of tropomyosin in nonmuscle cells

E Lazarides. J Cell Biol. 1975 Jun.

Abstract

An antibody against purified chicken skeletal muscle tropomyosin is used in indirect immunofluorescence to visualize the localization of tropomyosin in a variety of nonmuscle cells. The antibody produces a fluorescent pattern which is very similar to that obtained with an actin-specific antibody. This pattern is composed of fluorescent fibers which are shown to be coincident with the fibers seen with phase-contrast optics. High resolution epifluorescent microscopy reveals that fibers stained with the actin antibody show a continuous fluorescence, while fibers reacted with the tropomyosin antibody show a periodic fluorescence. Measurements indicate that the lengths of the fluorescent segments are variable with an average of 1.2 mum while the spacing between segments is approximately 0.4 mum.

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References

    1. J Cell Biol. 1963 Nov;19:369-90 - PubMed
    1. J Mol Biol. 1963 Sep;7:281-308 - PubMed
    1. Nature. 1972 Apr 14;236(5346):350-1 - PubMed
    1. J Ultrastruct Res. 1971 Nov;37(3):351-69 - PubMed
    1. J Cell Biol. 1966 Mar;28(3):505-25 - PubMed

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