Rat alpha-fetoprotein promoter and proximal enhancer direct expression of the nls-lacZ reporter gene in embryonic yolk sac, gut, and eustachian canal of transgenic mice

Abstract

The alpha-fetoprotein (AFP) gene is expressed mainly in the yolk sac, liver and intestine during embryonic and fetal life. We have analyzed the activities of some of the rat AFP regulatory elements in vivo, using transgenic mice bearing the LacZ gene with a nuclear localization signal (nls-lacZ) placed under the control of the rat AFP promoter and the most proximal enhancer regions (from -3,127 to +102). Four of the six transgenic lines, with two genetic backgrounds, had highly specific reproducible patterns of transgene expression on embryonic days E10.5, E12 and E15. Analyses were performed on the whole embryo and histologically. There was nuclear staining in the yolk sac endodermal cells and in the epithelial cells of the intestine, indicating that the proximal enhancer and promoter drive expression in these cells where the AFP gene is actively transcribed. The pharyngo-tympanic canal was also stained in the transgenic embryos. But there was no expression of the lacZ transgene in the embryonic liver, indicating that additional sequences of rat genomic DNA are required for correct expression in the liver.