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Comment
. 2000 Aug 15;97(17):9357-9.
doi: 10.1073/pnas.97.17.9357.

A large step for myosin

T Yanagida  1 , A H Iwane
Affiliations
Comment

A large step for myosin

T Yanagida et al. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Figure 1
Figure 1
Models of an actomyosin motor. (a) Tight coupling model. A typical tight coupling model is a lever-arm model. In this model, a lever-arm tilting (conformational change) is coupled to each ATPase cycle in a one-to-one fashion. Displacement per ATPase cycle is expected to be proportional to the length of a neck domain (lever arm). (b) Loose coupling model. Each mechanical event is not coupled to the ATPase cycle. A typical loose coupling model is a biased Brownian ratchet model, in which the myosin head thermally diffuses along an actin filament, say, according to asymmetric potentials (8). One of the two heads of myosin II is not drawn here, because the two heads are thought to operate independently. On other hand, the two heads of myosin V are expected to operate in a hand-over-hand fashion to travel long distances processively.
Figure 2
Figure 2
Direct observation of movement of single myosin V molecules along an actin filament (13). Myosin V is fluorescently labeled, exchanging one of the endogenous calmodulins bound to the neck domains by a fluorescently labeled one with Cy-3. Movement of single labeled myosin V molecules along an actin filament is observed by total internal reflection fluorescence microscopy (5). Red spots indicate Cy-3-labeled myosin V. An actin filament (green), which is labeled with rhodamine green phalloidine, is visualized separately and superimposed. Myosin V and an actin filament are artificially colored for convenience.
See this image and copyright information in PMC

Comment on

References

    1. Huxley H E. Science. 1969;164:1356–1366. - PubMed
    1. Huxley A F, Simmons R M. Nature (London) 1971;233:533–538. - PubMed
    1. Rief M, Rock R S, Mehta A D, Mooseker M S, Cheney R E, Spudich J A. Proc Natl Acad Sci USA. 2000;97:9482–9486. - PMC - PubMed
    1. Rayment I, Rypniewski W R, Schmidt-Base K, Smith R, Tomchick D R, Benning M M, Winkelmann D A, Wesenberg G, Holden H M. Science. 1993;261:50–58. - PubMed
    1. Ishii Y, Yanagida T. Single Mol. 2000;1:5–16.

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