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. 1975 Jun;72(6):2325-9.
doi: 10.1073/pnas.72.6.2325.

Ribosomal protein S1 and polypeptide chain initiation in bacteria

W Szer, J M Hermoso, S Leffler

Ribosomal protein S1 and polypeptide chain initiation in bacteria

W Szer et al. Proc Natl Acad Sci U S A. 1975 Jun.

Abstract

Among several subspecies of 30S subunits of Escherichia coli observed by polyacrylamide-agarose gel electrophoresis, only the slow-moving, protein S1-containing subspecies participates in the formation of the 30S initiation complex with coliphage MS2 RNA as mRNA; the other subspecies retain activity with AUG as mRNA; they are also active in the poly(U)-directed binding of Phe-tRNA. Protein S1 from Caulobacter crescentus substitutes for E. coli S1 despite the fact that C. crescentus ribosomes do not bind MS2 RNA. Under appropriate conditions, the entire population of E. coli 30S subunits can be isolated as the S1-containing subspecies. Protein S1 is lost by salt treatment of ribosomes.

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References

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