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. 1979 Mar;62(3):361-7.
doi: 10.3168/jds.s0022-0302(79)83252-x.

Thermal inactivation of a heat-resistant lipase produced by the psychotrophic bacterium Pseudomonas fluorescens

Thermal inactivation of a heat-resistant lipase produced by the psychotrophic bacterium Pseudomonas fluorescens

R E Andersson et al. J Dairy Sci. 1979 Mar.

Abstract

Lipase from Pseudomonas fluorescens was studied for thermostability at temperatures ranging from 100 C to 160 C. The heat treatments were in two media, and heating times necessary to inactivate 90% of the enzyme at constant temperature were extremely long even at high temperatures, e.g. 3.6 min at 140 C in nutrient broth and 2.0 min at 170 C in skim milk. The increments of temperature to reduce these heating times 90% were 37.0 C in nutrient broth and 38.9 C in skim milk. The lipase was inactivated only partly after 20 h at 20 C in 8 M urea, 6 M guanidine hydrochloride, and 1.0% sodium dodecyl sulfate. Four percent 2-mercaptoethanol showed no effect.

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