Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Aug 18;289(5482):1188-90.
doi: 10.1126/science.289.5482.1188.

Pig cloning by microinjection of fetal fibroblast nuclei

A Onishi  1 , M Iwamoto, T Akita, S Mikawa, K Takeda, T Awata, H Hanada, A C Perry
Affiliations

Pig cloning by microinjection of fetal fibroblast nuclei

A Onishi et al. Science. .

Abstract

Pig cloning will have a marked impact on the optimization of meat production and xenotransplantation. To clone pigs from differentiated cells, we microinjected the nuclei of porcine (Sus scrofa) fetal fibroblasts into enucleated oocytes, and development was induced by electroactivation. The transfer of 110 cloned embryos to four surrogate mothers produced an apparently normal female piglet. The clonal provenance of the piglet was indicated by her coat color and confirmed by DNA microsatellite analysis.

PubMed Disclaimer

Comment in

Publication types

LinkOut - more resources