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. 2000 Sep;68(9):5466-8.
doi: 10.1128/IAI.68.9.5466-5468.2000.

Presence of phosphorylcholine on a filarial nematode protein influences immunoglobulin G subclass response to the molecule by an interleukin-10-dependent mechanism

K M Houston  1 , E H Wilson, L Eyres, F Brombacher, M M Harnett, J Alexander, W Harnett
Affiliations

Presence of phosphorylcholine on a filarial nematode protein influences immunoglobulin G subclass response to the molecule by an interleukin-10-dependent mechanism

K M Houston et al. Infect Immun. 2000 Sep.

Abstract

The filarial nematode product ES-62 contains phosphorylcholine (PC) covalently attached to N-linked glycans. ES-62 induced high levels of immunoglobulin G1 (IgG1) antibodies, but no IgG2a, to non-PC epitopes of the molecule following subcutaneous injection into BALB/c mice. Conversely, mice given ES-62 lacking PC demonstrated significant production of both IgG subclasses. Thus, PC appears to block production of IgG2a antibodies to other epitopes on the parasite molecule. A role for interleukin-10 (IL-10) in this effect was shown by the ability of IL-10(-/-) mice to make an IgG2a antibody response to non-PC epitopes of ES-62.

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Figures

FIG. 1
FIG. 1
Comparison of IgG1 (A) and IgG2a (B) levels in BALB/c mice exposed to either ES-62 or ES-62 synthesized in the presence of dMM. Specific IgG2a titers were significantly higher in mice inoculated with ES-62 synthesized in the presence of dMM (P < 0.05; Mann-Whitney U test). Values represent the mean reciprocal end point dilution ± standard error (n = 4). The same result was obtained when HC-3 rather than dMM was employed to produce PC-free ES-62.
FIG. 2
FIG. 2
Analysis of IgG2a anti-BSA antibody titers in BALB/c mice exposed to either PC-BSA or BSA. Antibody titers are higher in mice exposed to BSA (P < 0.01; Mann-Whitney U test). Results are expressed as mean reciprocal end point dilutions ± standard error (n = 4).
FIG. 3
FIG. 3
(A) Comparison of the development of specific IgG2a in IL-4+/+ (wild type), IL-4−/−, and IL-4Rα−/− mice following 5 weeks of exposure to ES-62. Values represent the mean reciprocal end point dilution ± standard error (n = 4). (B) Comparison of the development of IgG2a in IL-10−/− and IL-10+/+ mice following 5 weeks of exposure to ES-62. Specific IgG2a titers were significantly higher in IL-10−/− mice (P < 0.025; Mann-Whitney U test). Values represent the mean reciprocal end point dilution ± standard error (n = 4).
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