The immunohistolocalization of carbonic anhydrase in rodent tissues

Abstract

Carbonic anhydrase has been localized with an immunoenzyme bridge technique in the following sites in paraffin sections of fixed rodent tissues: gastric parietal cells, the brush border of enterocytes in the small intestine, superficial nongoblet cells of the colon, selective segments of the nephron, glial cells, erythrocytes and adipose cells. Immunocytochemical localizations of carbonic anhydrase isozymes I and II in different histologic sites, by means of affinity column purified antibodies, agreed with the distribution of these enzymes in the various sites, as indicated by immunologic assays. The immunocytochemical results are compared with those reported for the cobalt-bicarbonate cytochemical method and with biochemical knowledge of the occurence of carbonic anhydrase.