Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2000:476:225-36.
doi: 10.1007/978-1-4615-4221-6_18.

Quantitative assays for the chick chorioallantoic membrane

Affiliations
Review

Quantitative assays for the chick chorioallantoic membrane

W D Thompson et al. Adv Exp Med Biol. 2000.

Abstract

Focal application of angiogenic substances to the chick chorioallantoic membrane is quick and easy as a rapid screening test, but is susceptible to artefactual stimulation induced by carriers, hyperosmolarity, proteolytic activity, and indeed any cause of damage to the CAM. This can be deceptive and unanticipated. Focal application methods can be used for subsequent measurement by morphometry of the increased vascularity forming the typical spokewheel pattern of supply vessels. If test and control substances are applied in liquid form to the whole dropped CAM surface, then a much wider variety of quantitative morphometric, histological and biochemical techniques can be applied. Assessment of arterial vascularity, terminal arterial branching, supply vessels in cross-sections, and CAM haemoglobin content are direct measures of angiogenic effects, but are time-consuming. Biochemical assays of collagen, protein and DNA synthesis parallel the other assays, and these parameters can be estimated more quickly within the working week. There is inherent variability in the outbred strains of hen eggs currently commercially available. This means that all assays require substantial group numbers to achieve statistical validity, generally not less than 10 eggs per group. The biochemical assays yield interesting time-course patterns that distinguish between different types of angiogenic stimulants.

PubMed Disclaimer

Publication types

LinkOut - more resources