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. 2000 Sep;44(9):2581-4.
doi: 10.1128/AAC.44.9.2581-2584.2000.

Mutant prevention concentration as a measure of antibiotic potency: studies with clinical isolates of Mycobacterium tuberculosis

Y Dong  1 , X Zhao, B N Kreiswirth, K Drlica
Affiliations

Mutant prevention concentration as a measure of antibiotic potency: studies with clinical isolates of Mycobacterium tuberculosis

Y Dong et al. Antimicrob Agents Chemother. 2000 Sep.

Abstract

The mutant prevention concentration (MPC) of a C-8-methoxy fluoroquinolone exhibited a narrow distribution for 14 genetically diverse clinical isolates of Mycobacterium tuberculosis, indicating that results from single-isolate studies are likely to be representative. When one isolate was challenged with a variety of antituberculosis agents, C-8-methoxy fluoroquinolones were exceptional in having MPCs below the maximum concentration attained in serum by use of commonly recommended doses.

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Figures

FIG. 1
FIG. 1
Fluoroquinolone structures.
FIG. 2
FIG. 2
Effect of fluoroquinolone concentration on selection of resistant mutants. M. tuberculosis isolate TN7804 was applied to agar plates containing the indicated concentrations of PD161148 (C-8-methoxy; open circles) or PD160793 (C-8-H; filled circles). After incubation, the number of drug-resistant colonies was recorded and plotted relative to the number of CFU applied to the drug-free agar plates. The arrowhead indicates the drug concentration at which no colony was recovered from plates containing C-8-methoxy fluoroquinolone when more than 1010 cells were applied. (Inset) Data for points where the fraction of cells recovered was below 10−7, replotted using a linear scale.
FIG. 3
FIG. 3
Effect of antituberculosis agent concentration on selection of resistant mutants. M. tuberculosis isolate TN6515 was applied to agar plates containing the indicated concentrations of isoniazid (A), rifampin (B), streptomycin (C), and ciprofloxacin (D). After incubation, the number of drug-resistant colonies was recorded and was plotted relative to the number of CFU applied to drug-free agar plates.
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