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Comment
. 2000 Aug 21;150(4):F107-9.
doi: 10.1083/jcb.150.4.f107.

Rho GTPases. Integrating integrin signaling

A Ridley  1
Affiliations
Comment

Rho GTPases. Integrating integrin signaling

A Ridley. J Cell Biol. .
No abstract available

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Figures

Figure 1
Figure 1
Methods for analyzing Rho, Rac and Cdc42 involvement in cellular responses. (A) Rho GTPases cycle between an inactive, GDP-bound conformation and an active, GTP-bound conformation. Incoming signals stimulate an increase in GTP-bound Rho, by activating exchange factors (GEFs) and/or by inactivating GTPase activating proteins (GAPs). Dominant-negative mutants of Rho proteins (e.g., Rho-N19) bind to GEFs, preventing them from activating endogenous Rho. Constitutively active mutants (e.g., Rho-V14) are unable to hydrolyze GTP, and signal continuously to downstream targets. (B) Active Rho-GTP can be isolated from cell lysates by incubation with a hybrid protein consisting of glutathione-S-transferase (GST) fused to the Rho-binding domain (RBD) of a downstream target, such as Rhoteckin.
Figure 2
Figure 2
Involvement of Rho, Rac and Cdc42 in responses to ECM. Adhesion of cells to thrombospondin-1 (TSP-1) leads to activation of Rac and Cdc42, which are required for extension of fascin-containing microspikes and cell migration. TSP-1 effects on Rho are not known. Adhesion of cells to a composite matrix of tenascin-C/fibronectin (FN)/fibrin prevents Rho activation. Effects on Rac and Cdc42 have not been determined.
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References

    1. Adams J.C. Formation of stable microspikes containing actin and the 55 kDa actin bundling protein, fascin, is a consequence of cell adhesion to thrombospondin-1implications for the anti-adhesive activities of thrombospondin-1. J. Cell Sci. 1995;108:1977–1990. - PubMed
    1. Adams J.C. Thrombospondin-1 Int. J. Biochem. Cell Biol. 29 1997. 861 865a - PubMed
    1. Adams J.C. Characterization of cell-matrix adhesion requirements for the formation of fascin microspikes Mol. Biol. Cell. 8 1997. 2345 2363b - PMC - PubMed
    1. Adams J.C., Schwartz M.A. Stimulation of fascin microspikes by thrombospondin-1 is mediated by the GTPases Rac and Cdc42. J. Cell Biol. 2000;150:807–822. - PMC - PubMed
    1. Barry S.T., Flinn H.M., Humphries M., Critchley D.R., Ridley A.J. Requirement for Rho in integrin signalling. Cell Adhes. Commun. 1997;4:387–398. - PubMed