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. 1975 Jul;123(1):20-7.
doi: 10.1128/jb.123.1.20-27.1975.

Isolation of a nontransmissible antibiotic resistance plasmid by transductional shortening of R factor RP1

P L Shipley, R H Olsen

Isolation of a nontransmissible antibiotic resistance plasmid by transductional shortening of R factor RP1

P L Shipley et al. J Bacteriol. 1975 Jul.

Abstract

A plasmid segregant carrying tetracycline and carbenicillin resistance markers has been isolated from R factor RP1 by transductional shortening with phage P22. The new plasmid RP1-S2, which has a molecular weight of 23 times 10-6, has lost the transfer, phage sensitivity, and neomycin resistance functions of RP1. It combines readily with a W group plasmid, R388, to form a transmissible carbenicillin and trimethoprim resistance plasmid, RWP1.

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References

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