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. 2000 Aug;86(4):857-9.
doi: 10.1645/0022-3395(2000)086[0857:DOTGBP]2.0.CO;2.

Detection of Toxoplasma gondii by polymerase chain reaction in sera of acutely infected mice

J Hafid  1 , D Guichard, P Flori, T Bourlet, H Raberin, C Genin, R T Sung
Affiliations

Detection of Toxoplasma gondii by polymerase chain reaction in sera of acutely infected mice

J Hafid et al. J Parasitol. 2000 Aug.

Abstract

The presence of Toxoplasma gondii DNA was detected in sera of acutely infected mice by polymerase chain reaction. Adult mice were inoculated intraperitoneally with 5 x 10(3) T. gondii RH strain tachyzoites. Five mice were killed every 3 hr from 3 to 21 hr post infection (PI) and every day from 1 to 7 days PI. Toxoplasma gondii DNA was first detected in 60% of the infected mice 18 hr PI and in 100% of the animals 21 hr PI and from 1 to 7 days PI. No mice survived longer than 7 days.

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