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. 1975 Feb 28;55(1):5-8.
doi: 10.1007/BF00467082.

[Antibiosis shown by a strain of Byssochlamys nivea Westling, 1909. III. Chromatographic characteristics and isolation of the active fraction]

[Article in French]
A M Colin, G Percebois

[Antibiosis shown by a strain of Byssochlamys nivea Westling, 1909. III. Chromatographic characteristics and isolation of the active fraction]

[Article in French]
A M Colin et al. Mycopathologia. .

Abstract

Early papers revealed that culture filtrate of Byssochlamys nivea has antibiotic properties. Active filtrate of B. nivea is extracted with ethyl acetate. Paper chromatography is carried out on Whatman no. 1; thin-layer chromatography is carried out on Kieselgel; visualization with bioautographic technique reveals a single spot. Ethyl acetate extract is passed through silicic acid-celite column. Each isolated fraction is tested for antibiotic activity. Only one supports this activity; a crystallized matter, melting point 109-110 degrees C, thermostable, is obtained.

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