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. 1975 Jun 12;255(5509):533-8.
doi: 10.1038/255533a0.

Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli

L H Kedes, A C Chang, D Houseman, S N Cohen

Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli

L H Kedes et al. Nature. .

Abstract

Histone genes have been cloned selectively in Escherichia coli directly from unfractionated sea urchin DNA using labelled mRNA probe. The cloning method used is potentially applicable for isolating any genetic sequence for which a probe is available. The several eukaryotic gene fragments introduced into bacteria by this method collectively contain all or nearly all of the DNA sequences represented in histone mRNA.

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