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. 2000 Sep;182(18):5274-7.
doi: 10.1128/JB.182.18.5274-5277.2000.

Mutational analysis of genes encoding the early flagellar components of Helicobacter pylori: evidence for transcriptional regulation of flagellin A biosynthesis

E Allan  1 , N Dorrell, S Foynes, M Anyim, B W Wren
Affiliations

Mutational analysis of genes encoding the early flagellar components of Helicobacter pylori: evidence for transcriptional regulation of flagellin A biosynthesis

E Allan et al. J Bacteriol. 2000 Sep.

Abstract

We investigated the roles of fliF, fliS, flhB, fliQ, fliG, and fliI of Helicobacter pylori, predicted by homology to encode structural components of the flagellar basal body and export apparatus. Mutation of these genes resulted in nonmotile, nonflagellate strains. Western blot analysis showed that all the mutants had considerably reduced levels of both flagellin subunits and of FlgE, the flagellar hook protein. RNA slot blot hybridization showed reduced levels of flaA mRNA, indicating that transcription of the major flagellin gene is inhibited in the absence of the early components of the flagellar-assembly pathway. This is the first demonstration of a checkpoint in H. pylori flagellar assembly.

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Figures

FIG. 1
FIG. 1
Western blot of whole-cell lysates of wild-type H. pylori (SS1) (lane 1) and fliI (lane 2), fliG (lane 3), fliQ (lane 4), flhB (lane 5), fliS (lane 6), and fliF (lane 7) mutants.
FIG. 2
FIG. 2
Slot blot hybridization of total RNAs with probes specific for either 16S rRNA (top), to determine that equal amounts of RNA were loaded, or flaA mRNA (bottom). Tests were performed on H. pylori SS1 (wild-type strain) (lane 1) and fliS (lane 2), fliF (lane 3), fliI (lane 4), fliG (lane 5), fliQ (lane 6), and flhB (lane 7) mutants. Hybridization was detected by phosphorimaging with an exposure of 72 h.
See this image and copyright information in PMC

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