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. 1975 Jun;2(6):865-71.
doi: 10.1093/nar/2.6.865.

Specific cleavage of tRNA by nuclease S1

F Harada, J E Dahlberg

Specific cleavage of tRNA by nuclease S1

F Harada et al. Nucleic Acids Res. 1975 Jun.

Abstract

Nuclease S1 specifically hydrolizes tRNAs in their anticodon loops, forming new 5' phosphate and 3' OH ends. Some single-stranded regions are not cut by nuclease S1. The strong preference of nuclease S1 for the anticodon region can be used for rapid identification of an anticodon-containing oligonucleotide and subsequent identification of the probable amino acid specificity of tRNA.

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