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. 2000 Sep;38(9):3399-403.
doi: 10.1128/JCM.38.9.3399-3403.2000.

Characterization of Actinomyces isolates from infected root canals of teeth: description of Actinomyces radicidentis sp. nov

M D Collins  1 , L Hoyles, S Kalfas, G Sundquist, T Monsen, N Nikolaitchouk, E Falsen
Affiliations

Characterization of Actinomyces isolates from infected root canals of teeth: description of Actinomyces radicidentis sp. nov

M D Collins et al. J Clin Microbiol. 2000 Sep.

Abstract

Two strains of a previously undescribed Actinomyces-like bacterium were recovered in pure culture from infected root canals of teeth. Analysis by biochemical testing and polyacrylamide gel electrophoresis of whole-cell proteins indicated that the strains closely resembled each other phenotypically but were distinct from previously described Actinomyces and Arcanobacterium species. Comparative 16S rRNA gene-sequencing studies showed the bacterium to be a hitherto unknown subline within a group of Actinomyces species which includes Actinomyces bovis, the type species of the genus. Based on phylogenetic and phenotypic evidence, we propose that the unknown bacterium isolated from human clinical specimens be classified as Actinomyces radicidentis sp. nov. The type strain of Actinomyces radicidentis is CCUG 36733.

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Figures

FIG. 1
FIG. 1
Similarity dendrogram based on whole-cell protein patterns of A. radicidentis sp. nov. and related species. Levels of correlation are expressed as percentages of similarity for convenience.
FIG. 2
FIG. 2
Unrooted tree showing the phylogenetic relationships of A. radicidentis sp. nov and some other high-G+C content gram-positive bacteria. The tree constructed using the neighbor-joining method was based on a comparison of approximately 1,327 nucleotides. Bootstrap values, expressed as percentages of 500 replications, are given at branching points. Bar, 1% sequence divergence.
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