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Comparative Study
. 2000 Sep;7(5):739-44.
doi: 10.1128/CDLI.7.5.739-744.2000.

Comparison of five serologic tests for diagnosis of acute infections by Chlamydia pneumoniae

K Persson  1 , J Boman
Affiliations
Comparative Study

Comparison of five serologic tests for diagnosis of acute infections by Chlamydia pneumoniae

K Persson et al. Clin Diagn Lab Immunol. 2000 Sep.

Abstract

Serology is often used to diagnose acute infections by Chlamydia pneumoniae. In this study paired sera from patients with acute respiratory tract infection during an epidemic of C. pneumoniae infections were examined by five different antibody tests. These tests were the complement fixation (CF) test, the microimmunofluorescence (MIF) test, a recombinant enzyme immunoassay (rEIA) (Medac) based on a recombinant lipopolysaccharide of chlamydia and measuring antibodies to a common chlamydial antigen, and two tests that utilize preparations of C. pneumoniae organisms, the SeroCp-EIA (Savyon) (with preserved lipopolysaccharide) and the LOY-EIA (Labsystems) (without this antigen). Both of the last two tests should measure specific antibodies to C. pneumoniae, although cross-reacting antibodies may also be detected by the SeroCp-EIA. Acute infection of C. pneumoniae was serologically confirmed in 44% of the cases by at least two different tests. Using an expanded "gold standard," i.e., the presence of significant reactions in at least two tests, the sensitivity of the CF test was 69%, that of the MIF test was 88%, that of the rEIA was 89%, that of the LOY-EIA was 96%, and that of the SeroCp-EIA was 92%. Specificity was high for all methods, but adjustments of diagnostic criteria were made to several of the tests. The basis for these adjustments and supportive data are presented. Infections of C. pneumoniae were detected in patients from 8 to 83 years of age. Two peaks in the incidence of such infections were observed: one among young teenagers and a second in adults 30 to 45 years of age, corresponding to parents of young teen-agers. The tests were equally sensitive in different age groups. Reinfections seemed to be rare.

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Figures

FIG. 1
FIG. 1
Rates of serologically confirmed cases of acute infections by C. pneumoniae in different age groups.
See this image and copyright information in PMC

References

    1. Aldous M B, Grayston J T, Wang S P, Foy H M. Seroepidemiology of Chlamydia pneumoniae TWAR infection in Seattle families, 1966–1979. J Infect Dis. 1992;166:646–649. - PubMed
    1. Berdal B P, Scheel O, Ögaard A R, Hoel T, Gutteberg T J, Ånestad G. Spread of subclinical Chlamydia pneumoniae infection in a closed community. Scand J Infect Dis. 1992;24:431–436. - PubMed
    1. Black C M, Fields P I, Messmer T O, Berdal B P. Detection of Chlamydia pneumoniae in clinical specimens by polymerase chain reaction using nested primers. Eur J Clin Microbiol Infect Dis. 1994;13:752–756. - PubMed
    1. Boman J, Allard A, Persson K, Lundborg M, Juto P, Wadell G. Rapid diagnosis of respiratory Chlamydia pneumoniae infection by nested touchdown polymerase chain reaction compared with culture and antigen detection by EIA. J Infect Dis. 1997;175:1523–1526. - PubMed
    1. Bourke S J, Carrington D, Frew C E, Stevenson R D, Banham S W. Serological cross-reactivity among chlamydial strains in a family outbreak of psittacosis. J Infect. 1989;19:41–45. - PubMed

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