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Multicenter Study
. 2000 Sep;7(5):759-63.
doi: 10.1128/CDLI.7.5.759-763.2000.

Shipment impairs lymphocyte proliferative responses to microbial antigens

R A Betensky  1 , E Connick, J Devers, A L Landay, M Nokta, S Plaeger, H Rosenblatt, J L Schmitz, F Valentine, D Wara, A Weinberg, H M Lederman
Affiliations
Multicenter Study

Shipment impairs lymphocyte proliferative responses to microbial antigens

R A Betensky et al. Clin Diagn Lab Immunol. 2000 Sep.

Abstract

Lymphocyte proliferation assays (LPAs) are widely used to assess T-lymphocyte function of patients with human immunodeficiency virus infection and other primary and secondary immunodeficiency disorders. Since these assays require expertise not readily available at all clinical sites, specimens may be shipped to central labs for testing. We conducted a large multicenter study to evaluate the effects of shipping on assay performance and found significant loss of LPA activity. This may lead to erroneous results for individual subjects and introduce bias into multicenter trials.

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Figures

FIG. 1
FIG. 1
Comparison of LPA data from fresh versus shipped cells (A to C) and from fresh versus bench cells (D to F). Results are expressed as log SI (median counts per minute for stimulated wells/median counts per minute for control wells). The x axis represents the log SI for the shipped or bench assays, and the y axis represents the log SI for the fresh assays. Deviations of the points from the 45° line depicted in each panel indicate a lack of agreement between the log SI from fresh assays and the log SI from shipped or bench assays. SK, streptokinase.
FIG. 2
FIG. 2
Comparison of LPA data from fresh versus shipped cells (A to D) and from fresh versus bench cells (E to H). Results are expressed as log counts per minute. The x axis represents the log counts per minute for the shipped or bench assays, and the y axis represents the log counts per minute for the fresh assays. Deviations of the points from the 45° line depicted in each panel indicate a lack of agreement between the log counts per minute from fresh assays and the log counts per minute from shipped or bench assays. SK, streptokinase.
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