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. 2000 Sep;7(5):774-7.
doi: 10.1128/CDLI.7.5.774-777.2000.

Serotype-cross-reactive immunoglobulin M responses in dengue virus infections determined by enzyme-linked immunosorbent assay

M Nawa  1 , K I Yamada, T Takasaki, T Akatsuka, I Kurane
Affiliations

Serotype-cross-reactive immunoglobulin M responses in dengue virus infections determined by enzyme-linked immunosorbent assay

M Nawa et al. Clin Diagn Lab Immunol. 2000 Sep.

Abstract

We developed immunoglobulin M (IgM) antibody capture enzyme-linked immunosorbent assays (ELISAs) with four monovalent dengue virus antigens. We attempted to determine whether IgM responses in dengue virus infections are serotype specific or serotype cross-reactive. Serum samples from 14 confirmed dengue cases were examined. In these 14 cases, which consisted of 12 Japanese and 2 non-Japanese patients, infecting dengue virus serotypes were defined by reverse transcription-PCR. Thirteen of the 14 cases were IgM positive in ELISA. IgM responses were serotype cross-reactive in these 13 cases but were highest against infecting dengue virus serotype in 9 of the 13 cases. These results indicate that IgM responses are generally dengue serotype cross-reactive but that IgM levels are highest against the infecting serotype in most dengue cases.

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Figures

FIG. 1
FIG. 1
Titration of dengue viral antigens by a sandwich ELISA. Prototype dengue virus type 1 (●)-, type 2 (⋄)-, type 3 (▪)-, and type 4 (▴)-infected C6/36 cell culture fluids were diluted with uninfected control culture fluid as the diluent.
FIG. 2
FIG. 2
Index values obtained in ELISA using tetravalent dengue virus antigen at various dilutions. The tetravalent dengue virus antigen (Ag.) was used at dilutions of 1:1, 1:2, and 1:4. Sera from 33 healthy Japanese adults (●) and 11 IgM-positive dengue patients (▫) and four reference sera given in the commercial kit (○) were used in the assay. H, L, C, and N, high IgM-positive, low IgM-positive, cutoff calibrator, and IgM-negative sera for the commercial ELISA kit, respectively.
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