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. 1975 Aug;123(2):687-92.
doi: 10.1128/jb.123.2.687-692.1975.

alphabeta sequence of F is IS31

S Hu, K Ptashne, S N Cohen, N Davidson

alphabeta sequence of F is IS31

S Hu et al. J Bacteriol. 1975 Aug.

Abstract

Previous studies have shown that there is a deoxyribonucleic acid (DNA) segment, of length 1.3 kb and denoted as the alphabeta sequence, which occurs twice on the F plasmid at corrdinates 93.2 to 94.5/OF kb and 13.7 to 15.0F kb. In the present investigation, heteroduplexes were prepared between a phage DNA carrying the insertion sequence IS3 and suitable F-prime DNAs. The hybrids formed show that IS3 is the same as alphabeta. This result plus previous studies support the view that: (i) the insertion sequence IS2 and IS3 occur on F and, in multiple copies, on the main bacterial chromosome of Escherichia coli K-12; and (ii)these IS sequences on the main bacterial chromosomes are hot spots for Hfr formation by reciprocal recombination with the corresponding sequences of F.

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References

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