Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 May;17(5):276-83.
doi: 10.1023/a:1009462400708.

FISH analysis of six chromosomes in unfertilized human oocytes after polar body removal

E Martini  1 , S P Flaherty, N J Swann, C D Matthews, F C Ramaekers, J P Geraedts
Affiliations

FISH analysis of six chromosomes in unfertilized human oocytes after polar body removal

E Martini et al. J Assist Reprod Genet. 2000 May.

Abstract

Purpose: To develop an improved technique for estimating chromosomal abnormalities in human oocytes by fluorescence in situ hybridization (FISH) and to correlate the position of single chromatids with the chromosomal status of the oocytes.

Methods: Oocytes that were at metaphase II about 17-20 hr after insemination or intracytoplasmic sperm injection (ICSI) were treated with pronase to remove the zona pellucida and polar body (PB) and then spread on slides using HCl and Tween 20. Two rounds of FISH were performed using direct-labeled probes: chromosomes 1, 13, 21 (round 1); chromosomes X, 7, 18 (round 2).

Results: Of the 63 oocytes from 18 patients (mean age, 32 years), 48 (76%) had one DNA complement as expected, 9 (14%) had 2 DNA complements, 3 (5%) gave incomplete FISH signals, and 3 (5%) were not analyzable. Of the 48 oocytes with one set of DNA, 48% were haploid, 44% were aneuploid for one or more chromosomes, and 8% were polyploid. We also found an increased frequency of predivision of chromatid bivalents in aneuploid oocytes, especially for chromosome 21.

Conclusions: This technique enables simultaneous assessment of six chromosomes in human oocytes, and therefore can be useful for accurately determining the incidence and causes of genetic imbalances in human oocytes and apparently low fertilization rates.

PubMed Disclaimer

References

    1. Almeida PA, Bolton VN. The relationship between chromosomal abnormalities in the human oocyte and fertilization in vitro. Hum Reprod. 1994;9:343–346. - PubMed
    1. Grossmann M, Calafell JM, Moreno V, et al. Recurrent in vitro fertilization failure evaluated by fluorescence in situ hybridization: A case report. Fertil Steril. 1998;69:558–560. - PubMed
    1. Warburton D. Human female meiosis: new insights into an error-prone process. Am J Hum Genet. 1997;61:1–4. - PMC - PubMed
    1. Plachot M, Veiga A, Montagut J, et al. Are clinical and biological IVF parameters correlated with chromosomal disorders in early life: a multicentric study. Hum Reprod. 1988;3:627–635. - PubMed
    1. Benkhalifa D, Geneix A, Janny L, et al.: Chromosome aberrations human oocytes. In Fifth International Congress on Early Fetal Diagnosis

LinkOut - more resources