Effects of feeding on extracellular levels of glutamate in the medial and lateral portion of the globus pallidus of freely moving rats

Abstract

The globus pallidus (GP) is considered to be part of the basal ganglia and previous research has determined that it might be involved in feeding behavior. For example, it has been shown that the GP becomes active during feeding and that disinhibition of this nucleus, by locally injecting a GABA antagonist, is sufficient to induce feeding in a satiated rat. However, few studies have measured extracellular levels of glutamate during free feeding in the GP of rats. For this reason brain microdialysis coupled to capillary electrophoresis with laser-induced fluorescence was used to determine FTC-glutamate levels, either in the medial or lateral portion of the GP, of freely moving rats. Retrograde labeling of the neurons projecting to the two areas was also examined in an attempt to gain some insight on the identity of the neurons that released glutamate in the GP during feeding. Extracellular levels of glutamate-FTC differentially increased in both portions of the GP during a 2-min interval of free feeding. Retrograde labeling also showed that both areas received projections from different brain areas suggesting that each of the GP portions could be involved in separate aspects of the feeding behavior.