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. 1979 May;76(5):2381-4.
doi: 10.1073/pnas.76.5.2381.

Reevaluation of level of genic heterozygosity in natural population of Drosophila melanogaster by two-dimensional electrophoresis

A J Brown, C H Langley

Reevaluation of level of genic heterozygosity in natural population of Drosophila melanogaster by two-dimensional electrophoresis

A J Brown et al. Proc Natl Acad Sci U S A. 1979 May.

Abstract

We have found the two-dimensional electrophoretic technique of O'Farrell to be highly efficient in the detection of charge-change substitutions in a large number of proteins. We have applied this method to determine the level of heterozygosity of the most abundant proteins in Drosophila melanogaster adults from a natural population. The estimate of per-locus heterozygosity obtained from approximately 54 loci screened was 4% with 6 loci polymorphic. This is much lower than overall estimates obtained by standard gel electrophoresis but is not different from estimates for "Group I" enzymes--i.e., those utilizing a narrow spectrum of substrates of intracellular origin. We consider these data to throw open the question of the level of genetic variability in nature.

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