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. 2000 Oct;44(10):2684-8.
doi: 10.1128/AAC.44.10.2684-2688.2000.

Molecular characterization of integrons in Acinetobacter baumannii: description of a hybrid class 2 integron

M C Ploy  1 , F Denis, P Courvalin, T Lambert
Affiliations

Molecular characterization of integrons in Acinetobacter baumannii: description of a hybrid class 2 integron

M C Ploy et al. Antimicrob Agents Chemother. 2000 Oct.

Abstract

Twenty Acinetobacter baumannii strains resistant to various antibiotics were analyzed for integron content and sequences of the amplification products. Sixteen clinical isolates had a class 1 integron, 2 contained an additional class 1 or class 2 integron, but no class 3 integron was detected. Thirteen strains had integrons with a single cassette: aac(3)-Ia (9 strains), ant(2")-Ia (2 strains), or aac(6')-Ib (2 strains); 1 had aac(6')-Ib and oxa20 cassettes and an unknown gene; and 1 had an integron containing ant(2")-Ia and an oxa3 cassette truncated by IS6100. The remaining strains harbored class 1 integrons with gene cassettes previously found in Enterobacteriaceae. One integron had a hybrid structure composed of intI2 and the 3' conserved segment of class 1 integrons. These data indicate that integrons play a major role in multidrug resistance in Acinetobacter.

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Figures

FIG. 1
FIG. 1
Physical maps of the class 1 integron of BM4430 (A) and of the hybrid integron of BM4431 (B). Filled circle, attC site; open arrows, coding sequences; arrowheads, primers.
See this image and copyright information in PMC

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