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. 2000 Oct;44(10):2777-83.
doi: 10.1128/AAC.44.10.2777-2783.2000.

Animal and human multidrug-resistant, cephalosporin-resistant salmonella isolates expressing a plasmid-mediated CMY-2 AmpC beta-lactamase

P L Winokur  1 , A Brueggemann, D L DeSalvo, L Hoffmann, M D Apley, E K Uhlenhopp, M A Pfaller, G V Doern
Affiliations

Animal and human multidrug-resistant, cephalosporin-resistant salmonella isolates expressing a plasmid-mediated CMY-2 AmpC beta-lactamase

P L Winokur et al. Antimicrob Agents Chemother. 2000 Oct.

Abstract

Salmonella spp. are important food-borne pathogens that are demonstrating increasing antimicrobial resistance rates in isolates obtained from food animals and humans. In this study, 10 multidrug-resistant, cephalosporin-resistant Salmonella isolates from bovine, porcine, and human sources from a single geographic region were identified. All isolates demonstrated resistance to cephamycins and extended-spectrum cephalosporins as well as tetracycline, chloramphenicol, streptomycin, and sulfisoxazole. Molecular epidemiological analyses revealed eight distinct chromosomal DNA patterns, suggesting that clonal spread could not entirely explain the distribution of this antimicrobial resistance phenotype. However, all isolates encoded an AmpC-like beta-lactamase, CMY-2. Eight isolates contained a large nonconjugative plasmid that could transform Escherichia coli. Transformants coexpressed cephalosporin, tetracycline, chloramphenicol, streptomycin, and sulfisoxazole resistances. Plasmid DNA revealed highly related restriction fragments though plasmids appeared to have undergone some evolution over time. Multidrug-resistant, cephalosporin-resistant Salmonella spp. present significant therapeutic problems in animal and human health care and raise further questions about the association between antimicrobial resistance, antibiotic use in animals, and transfer of multidrug-resistant Salmonella spp. between animals and man.

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Figures

FIG. 1
FIG. 1
PFGE of XbaI-digested chromosomal DNA of Salmonella isolates. Isolates 912993, 12386, and 16033 are previously characterized Salmonella serovar Typhimurium DT104 isolates (13). MWM, molecular weight standard (a λ DNA ladder containing concatamers of the 48.5-kb phage DNA).
FIG. 2
FIG. 2
PstI- or EcoRI-digested plasmid DNA isolated from the DH10B E. coli transformants.
See this image and copyright information in PMC

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