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Review
. 2000 Sep;29(3):548-50, 552-4, 556 passim.
doi: 10.2144/00293bi01.

A concise guide to cDNA microarray analysis

P Hegde  1 , R Qi, K Abernathy, C Gay, S Dharap, R Gaspard, J E Hughes, E Snesrud, N Lee, J Quackenbush
Affiliations
Free article
Review

A concise guide to cDNA microarray analysis

P Hegde et al. Biotechniques. 2000 Sep.
Free article

Abstract

Microarray expression analysis has become one of the most widely used functional genomics tools. Efficient application of this technique requires the development of robust and reproducible protocols. We have optimized all aspects of the process, including PCR amplification of target cDNA clones, microarray printing, probe labeling and hybridization, and have developed strategies for data normalization and analysis.

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