Mouse connexin37: gene structure and promoter analysis

Abstract

Connexin37 (Cx37) is a subunit gap junction protein which exhibits limited expression in only a few cell types, predominantly in endothelial cells and in the lung. To begin to analyze Cx37 expression, we isolated a 1.6 kb mouse Cx37 cDNA from a mouse lung cDNA library and isolated corresponding mouse genomic clones from a bacterial artificial chromosome library. Sequencing and comparison of these clones showed that the Cx37 gene contained a short first exon, an 1.0 kb single intron and a second exon containing the complete coding region and 3'-untranslated region (UTR). The 5'-UTR of the mouse cDNA showed 70% identity to that of human Cx37. Primer extension experiments performed using mouse lung RNA gave two bands of sizes consistent with the transcription start site predicted from the cDNA. Sequence analysis showed that the regions flanking exon I contained a consensus 'TATA box' 43 bp 5' from the transcription start site preceded by several putative transcription factor binding sites and a 282 bp truncated L1Md interspersed element. Luciferase reporter gene transfections suggested that an area of 268 bp 5' from the first exon acted as a basal promoter for Cx37 and that there was a strong negative regulatory element in the intron.