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. 1975 Aug 14;256(5518):556-60.
doi: 10.1038/256556a0.

Multiple steps in DNA recognition by restriction endonuclease from E. coli K

R Yuan, T A Bickle, W Ebbers, C Brack

Multiple steps in DNA recognition by restriction endonuclease from E. coli K

R Yuan et al. Nature. .

Abstract

The process of DNA recognition by the activated form of the restriction endonuclease from E. coli K involves three enzyme-DNA complexes which can be differentiated experimentally. These are: an initial complex formed at a nonspecific site; a recognition complex involving the host specificity site; and a cleavage complex dependent on the presence of ATP.

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