Multiple steps in DNA recognition by restriction endonuclease from E. coli K
- PMID: 1101070
- DOI: 10.1038/256556a0
Multiple steps in DNA recognition by restriction endonuclease from E. coli K
Abstract
The process of DNA recognition by the activated form of the restriction endonuclease from E. coli K involves three enzyme-DNA complexes which can be differentiated experimentally. These are: an initial complex formed at a nonspecific site; a recognition complex involving the host specificity site; and a cleavage complex dependent on the presence of ATP.
Similar articles
-
Steps in the reaction mechanism of the Escherichia coli plasmid P15-specific restriction endonuclease.J Mol Biol. 1978 Jul 15;122(4):433-45. doi: 10.1016/0022-2836(78)90420-5. J Mol Biol. 1978. PMID: 357734 No abstract available.
-
ATP-induced conformational changes in the restriction endonuclease from Escherichia coli K-12.Proc Natl Acad Sci U S A. 1978 Jul;75(7):3099-103. doi: 10.1073/pnas.75.7.3099. Proc Natl Acad Sci U S A. 1978. PMID: 356045 Free PMC article.
-
The role of S-adenosylmethionine in the cleavage of deoxyribonucleic acid by the restriction endonuclease from Escherichia coli K.J Biol Chem. 1975 Jun 10;250(11):4159-64. J Biol Chem. 1975. PMID: 1092685
-
Structure and mechanism of multifunctional restriction endonucleases.Annu Rev Biochem. 1981;50:285-319. doi: 10.1146/annurev.bi.50.070181.001441. Annu Rev Biochem. 1981. PMID: 6267988 Review. No abstract available.
-
Structures and mechanisms of DNA restriction and modification enzymes.Q Rev Biophys. 1979 Aug;12(3):315-69. doi: 10.1017/s0033583500005461. Q Rev Biophys. 1979. PMID: 232555 Review. No abstract available.
Cited by
-
The use of heparin as a simple cost-effective means of controlling background in nucleic acid hybridization procedures.Nucleic Acids Res. 1984 Jul 25;12(14):5627-38. doi: 10.1093/nar/12.14.5627. Nucleic Acids Res. 1984. PMID: 6087294 Free PMC article.
-
An investigation of the structural requirements for ATP hydrolysis and DNA cleavage by the EcoKI Type I DNA restriction and modification enzyme.Nucleic Acids Res. 2011 Sep 1;39(17):7667-76. doi: 10.1093/nar/gkr480. Epub 2011 Jun 17. Nucleic Acids Res. 2011. PMID: 21685455 Free PMC article.
-
Promotion and limitation of genetic exchange.Experientia. 1979 Mar 15;35(3):287-93. doi: 10.1007/BF01964304. Experientia. 1979. PMID: 376329 Review. No abstract available.
-
DNA Walkers for Biosensing Development.Adv Sci (Weinh). 2022 Jun;9(18):e2200327. doi: 10.1002/advs.202200327. Epub 2022 Apr 22. Adv Sci (Weinh). 2022. PMID: 35460209 Free PMC article. Review.
-
The HsdR subunit of R.EcoR124II: cloning and over-expression of the gene and unexpected properties of the subunit.Nucleic Acids Res. 1997 Feb 1;25(3):503-11. doi: 10.1093/nar/25.3.503. Nucleic Acids Res. 1997. PMID: 9016588 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases